ARG42343
anti-acidic Cytokeratin antibody [AE1] (PE)
anti-acidic Cytokeratin antibody [AE1] (PE) for Flow cytometry and Human,Mouse,Rat,Cat,Chicken,Dog,Primates,Rabbit
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [AE1] recognizes acidic Cytokeratin |
|---|---|
| 反应物种 | Hu, Ms, Rat, Cat, Chk, Dog, NHuPrm, Rb |
| 应用 | FACS |
| 特异性 | Mouse monoclonal antibody AE1 recognizes acidic type cytokeratins (intracellular antigens), namely K10, 14, 15, 16, 19 (40-56 kDa). This antibody stains well the basal layer of epidermis and most epithelia. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | AE1 |
| 同位型 | IgG1 |
| 靶点名称 | acidic Cytokeratin |
| 抗原物种 | Human |
| 抗原 | Human epidermal keratins. |
| 偶联标记 | PE |
| 別名 | KPP; K10; CK-10; BIE; Keratin, type I cytoskeletal 10; Cytokeratin-10; CK10; BCIE; Keratin-10; EHK |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS and 15 mM Sodium azide. |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 0.1 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT10 |
| 全名 | keratin 10, type I |
| 背景介绍 | This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plays a role in the establishment of the epidermal barrier on plantar skin. (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization. (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur. [UniProt] |
| 细胞定位 | Secreted, extracellular space. Note=Localized on the surface of desquamated nasal epithelial cells. [UniProt] |
| 预测分子量 | 59 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG42343 anti-acidic Cytokeratin antibody [AE1] (PE) FACS image
Flow Cytometry: Separation of stained Caco-2 cells (red) from unstained Caco-2 cells (black). Cells were stained with ARG42343 anti-acidic Cytokeratin antibody [AE1] (PE) at 1 µg/ml dilution.
