ARG10186
anti-alpha Fetoprotein antibody [AFP-Y1]
anti-alpha Fetoprotein antibody [AFP-Y1] for ELISA,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody
概述
| 产品描述 | Mouse Monoclonal antibody [AFP-Y1] recognizes Human AFP |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, WB |
| 特异性 | No detectable cross-reaction with human normal serum proteins. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | AFP-Y1 |
| 同位型 | IgG2b |
| 靶点名称 | alpha Fetoprotein |
| 抗原物种 | Human |
| 抗原 | Human AFP, which was isolated from amniotic fluid and purified by affinity chromatography. |
| 偶联标记 | Un-conjugated |
| 別名 | Alpha-fetoprotein; HPAFP; FETA; Alpha-fetoglobulin; AFPD; Alpha-1-fetoprotein |
应用说明
| 应用说明 | ELISA: This antibody can be used as a capture antibody in sandwich immunoassay for human AFP detection. Western blot: The antibody identifies 68kD AFP on western blot. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein G affinity purified |
| 缓冲液 | 0.01M PBS (pH 7.0) |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AFP |
| 全名 | alpha-fetoprotein |
| 背景介绍 | Alpha fetoprotein (AFP) is a 70kD glycoprotein which is produced primarily by liver cells in foetuses. It exists in significant amount in foetal plasma and decreases rapidly after birth. Moderate amount of AFP exists in serum of pregnant women. Monitoring mother’s serum AFP level in second trimester pregnancy can help assessing birth defects such as Down syndrome, neural tube defect and anencephaly. Elevated AFP level in men, children and non-pregnant women is associated with neoplastic conditions in lever, testicle or ovary. |
| 生物功能 | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30065 Alpha Fetoprotein ELISA Antibody Duo Related products: alpha Fetoprotein antibodies; alpha Fetoprotein ELISA Kits; alpha Fetoprotein Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Stem cell and the regenerative medicine: Ready for the patients |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody |
| 预测分子量 | 69 kDa |
| 翻译后修饰 | Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated. |
检测图片 (1) Click the Picture to Zoom In
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ARG10186 anti-alpha Fetoprotein antibody [AFP-Y1] WB image
Western blot: 20 µl of HepG2 and MDA-MB-231 cell lysates stained with ARG10186 anti-alpha Fetoprotein antibody [AFP-Y1] at 1:500 dilution.
