ARG56134

anti-alpha Fetoprotein antibody

anti-alpha Fetoprotein antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

publication_link 参考文献1

概述

产品描述 Rabbit Polyclonal antibody recognizes alpha Fetoprotein
反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 alpha Fetoprotein
抗原物种 Human
抗原 Synthetic peptide (14 aa) within aa. 340-390 of Human alpha Fetoprotein.
偶联标记 Un-conjugated
別名 Alpha-fetoprotein; HPAFP; FETA; Alpha-fetoglobulin; AFPD; Alpha-1-fetoprotein

应用说明

应用建议
应用 推荐稀释比
ICC/IF20 µg/ml
IHC-P10 µg/ml
WB1 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Human liver tissue lysate

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS and 0.02% Sodium azide
抗菌剂 0.02% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 11576 Mouse AFP

GeneID: 174 Human AFP

Swiss-port # P02771 Human Alpha-fetoprotein

Swiss-port # P02772 Mouse Alpha-fetoprotein

基因名称 AFP
全名 alpha-fetoprotein
背景介绍 This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
生物功能 Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. [UniProt]
预测分子量 69 kDa
翻译后修饰 Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated.

检测图片 (5) Click the Picture to Zoom In

  • ARG56134 anti-alpha Fetoprotein antibody WB image

    Western blot: 20 µl of HepG2 and Mouse liver lysates stained with ARG56134 anti-alpha Fetoprotein antibody at 1 µg/ml dilution.

  • ARG56134 anti-alpha Fetoprotein antibody ICC/IF image

    Immunofluorescence: Rat liver tissue stained with ARG56134 anti-alpha Fetoprotein antibody at 20 µg/ml dilution.

  • ARG56134 anti-alpha Fetoprotein antibody IHC-P image

    Immunohistochemistry: Mouse liver tissue stained with ARG56134 anti-alpha Fetoprotein antibody at 2.5 µg/ml dilution.

  • ARG56134 anti-alpha Fetoprotein antibody WB image

    Western blot: fetal Human liver tissue lysate stained with ARG56134 anti-alpha Fetoprotein antibody at (A) 1 µg/ml and (B) 2 µg/ml dilution.

  • ARG56134 anti-alpha Fetoprotein antibody IHC-P image

    Immunohistochemistry: Rat liver tissue stained with ARG56134 anti-alpha Fetoprotein antibody at 10 µg/ml dilution.

文献引用

Chromosome territory reorganization through artificial chromosome fusion is compatible with cell fate determination and mouse development

IHC-P / Mouse

Yuang Wang et al.
Cell Discov.,  (2023)

publication_link

 

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