ARG67027
anti-alpha Fetoprotein antibody [SQab30312]
anti-alpha Fetoprotein antibody [SQab30312] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
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概述
| 产品描述 | Recombinant rabbit Monoclonal antibody [SQab30312] recognizes alpha Fetoprotein |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab30312 |
| 同位型 | IgG |
| 靶点名称 | alpha Fetoprotein |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human alpha Fetoprotein. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Alpha-fetoprotein |
| 別名 | AFP, Alpha Fetoprotein, Alpha-Fetoprotein, HPAFP , FETA, Alpha-1-Fetoprotein, Alpha-Fetoglobulin, AFPD |
应用说明
| 应用建议 |
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| 应用说明 | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Hepatocellular carcinoma | ||||||
| 实际分子量 | 65-72 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05%BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AFP |
| 全名 | Alpha Fetoprotein |
| 背景介绍 | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Oct 2019] |
| 生物功能 | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. [Uniprot] |
| 细胞定位 | Secreted |
| 预测分子量 | 69 kDa |
| 翻译后修饰 | Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. [Uniprot] |
检测图片 (2) Click the Picture to Zoom In
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ARG67027 anti-alpha Fetoprotein antibody [SQab30312] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded hepatocellular carcinoma stained with ARG67027 anti-alpha Fetoprotein antibody [SQab30312].
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ARG67027 anti-alpha Fetoprotein antibody [SQab30312] WB image (Customer review)
Western blot: HepG2 stained with ARG67027 anti-alpha Fetoprotein antibody [SQab30312] at 1:500 dilution.
客户反馈
Excellent
anti-alpha Fetoprotein antibody [SQab30312]
Application：WB
Sample：HepG2
Sample Loading Amount：10 μL
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
