ARG53696
anti-alpha Tubulin antibody
anti-alpha Tubulin antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Cow,Guinea pig,Gerbil,Pig,Xenopus laevis,Zebrafish
Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
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概述
| 产品描述 | Rabbit Polyclonal antibody recognizes alpha Tubulin |
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| 反应物种 | Hu, Ms, Rat, Cow, Gpig, Grb, Pig, Xenopus laevis, Zfsh |
| 应用 | ICC/IF, IHC-Fr, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | alpha Tubulin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from C-terminus of human tubulin alpha. |
| 偶联标记 | Un-conjugated |
| 別名 | TUBA1; ALS22; Tubulin alpha-4A chain; Testis-specific alpha-tubulin; Alpha-tubulin 1; Tubulin alpha-1 chain; Tubulin H2-alpha; H2-ALPHA |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Lung |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunogen affinity purified |
| 缓冲液 | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| 抗菌剂 | < 0.1% Sodium azide |
| 稳定剂 | 1% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TUBA4A |
| 全名 | tubulin, alpha 4a |
| 背景介绍 | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013] |
| 生物功能 | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProt] |
| 细胞定位 | Cytoplasm |
| 研究领域 | Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly. Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability. |
检测图片 (3) Click the Picture to Zoom In
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ARG53696 anti-alpha Tubulin antibody WB image
Western blot: 20 µg of 293T, Mouse brain and Rat brain lysates stained with ARG53696 anti-alpha Tubulin antibody at 1:10000 dilution.
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ARG53696 anti-alpha Tubulin antibody IHC-P image
Immunohistochemistry: Human Lung stained with ARG53696 anti-alpha Tubulin antibody
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ARG53696 anti-alpha Tubulin antibody WB image
Western blot: 30 µg of Mouse heart lysate stained with ARG53696 anti-alpha Tubulin antibody at 1:500 dilution.
客户反馈
Good
anti-alpha Tubulin antibody
Application：WB
Sample：293T, Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:10000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
