ARG65693
anti-alpha Tubulin antibody
anti-alpha Tubulin antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Signaling Transduction antibody; Loading Control antibody
参考文献13
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes alpha Tubulin |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | alpha Tubulin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human alpha Tubulin protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Tubulin K-alpha-1; Alpha-tubulin ubiquitous; Tubulin alpha-ubiquitous chain; Tubulin alpha-1B chain; K-ALPHA-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | NIH/3T3, Hela, HepG2, PC12, human stomach tissue, human tonsil tissue, mouse pancreas tissue |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 1*TBS (pH 7.4), 0.05% Sodium azide, 1% BSA and 40% Glycerol |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 1% BSA, 40% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TUBA1B |
| 全名 | tubulin, alpha 1b |
| 生物功能 | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30258 Loading Controls for Whole Cell Lysate Antibody Panel Related products: alpha Tubulin antibodies; alpha Tubulin Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Signaling Transduction antibody; Loading Control antibody |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly. Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability. Nitration of Tyr-451 is irreversible and interferes with normal dynein intracellular distribution. Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively. Tubulin alpha-1B chain: Tyrosination promotes microtubule interaction with CAP-Gly domain-containing proteins such as CLIP1, CLIP2 and DCTN1 (By similarity). Tyrosination regulates the initiation of dynein-dynactin motility via interaction with DCTN1, which brings the dynein-dynactin complex into contact with microtubules (PubMed:26972003). In neurons, tyrosinated tubulins mediate the initiation of retrograde vesicle transport (By similarity). Detyrosinated tubulin alpha-1B chain: Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator (PubMed:25908662). Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction (By similarity). |
检测图片 (11) Click the Picture to Zoom In
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ARG65693 anti-alpha Tubulin antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG65693 anti-alpha Tubulin antibody at 1:200 dilution. Left: primary antibody (orange). Middle: DAPI (blue). Right: Merge.
Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)
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ARG65693 anti-alpha Tubulin antibody WB image
Western blot: 20 µg of 293T, Mouse brain and Rat brain lysates stained with ARG65693 anti-alpha Tubulin antibody at 1:10000 dilution.
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ARG65693 anti-alpha Tubulin antibody WB image
Western blot: Mouse retina stained with ARG65693 anti-alpha Tubulin antibody and ARG65351 Goat anti-Rabbit IgG antibody (HRP).
From Xiaoyuan Ye et al. Mol Ther Nucleic Acids. (2024), doi: 10.1016/j.omtn.2024.102209, Fig. 5.D.
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ARG65693 anti-alpha Tubulin antibody WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG65693 anti-alpha Tubulin antibody at 1:5000 dilution.
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ARG65693 anti-alpha Tubulin antibody WB image
Western blot: 30 µg of 1) Mouse brain, and 2) Rat brain lysates stained with ARG65693 anti-alpha Tubulin antibody at 1:500 dilution.
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ARG65693 anti-alpha Tubulin antibody WB image
Western blot: A549 cells stained with ARG65693 anti-alpha Tubulin antibody at 1:10000 dilution.
From Youwei Huang et al. Oncol Rep (2022), doi: 10.3892/or.2022.8396, Fig. 4. A.
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ARG65693 anti-alpha Tubulin antibody WB image
Western blot: HCT116 cells stained with ARG65693 anti-alpha Tubulin antibody.
From Fang Wang et al. Cell Rep (2023), doi: 10.1016/j.celrep.2023.113318, Fig. 3. A.
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ARG65693 anti-alpha Tubulin antibody WB image
Western blot: Rat sample stained with ARG65693 anti-alpha Tubulin antibody at 1:5000 dilution.
From Jinzhi Li et al. J Reprod Immunol (2023), doi: 10.1016/j.jri.2023.104166, Fig. 2. B.
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ARG65693 anti-alpha Tubulin antibody WB image
Western blot: Glioma cells stained with ARG65693 anti-alpha Tubulin antibody at 1:5000 dilution.
From Xueqin Chen et al. Nat Commun (2023), doi: 10.1038/s41467-023-42545-3, Fig. 2. D.
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ARG65693 anti-alpha Tubulin antibody WB image
Western blot: HCT116 and SF763 cells stained with ARG65693 anti-alpha Tubulin antibody at 1:10000 dilution.
From Yuqing Yang et al. Oncol Rep (2024), doi: 10.3892/or.2024.8813, Fig. 6. B.
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ARG65693 anti-alpha Tubulin antibody WB image
Western blot: GBM cells stained with ARG65693 anti-alpha Tubulin antibody.
From Jialuo Mai et al. Mol Oncol (2019), doi: 10.1002/1878-0261.12525, Fig. 2. D.
客户反馈
Excellent
anti-alpha Tubulin antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：TRITC
Excellent
anti-alpha Tubulin antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：TRITC
Excellent
anti-alpha Tubulin antibody
Application：WB
Sample：293T, Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:10000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-alpha Tubulin antibody
Application：WB
Sample：
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
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