ARG52485
anti-alpha smooth muscle Actin antibody [1A4]
anti-alpha smooth muscle Actin antibody [1A4] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse,Pig,Rabbit,Rat,Sheep
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody
参考文献9
概述
| 产品描述 | Mouse Monoclonal antibody [1A4] recognizes alpha smooth muscle Actin (SMA) |
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| 反应物种 | Hu, Ms, Rat, Pig, Rb, Sheep |
| 应用 | ELISA, ICC/IF, IHC-Fr, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1A4 |
| 同位型 | IgG1, kappa |
| 靶点名称 | alpha smooth muscle Actin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide from N-terminus of human alpha smooth muscle isoform of actin. |
| 偶联标记 | Un-conjugated |
| 別名 | Cell growth-inhibiting gene 46 protein; MYMY5; ACTSA; Alpha-actin-2; Actin, aortic smooth muscle; AAT6 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Incubation time: overnight at 4°C or 30-60 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Leiomyoma, Small Intestine (Smooth Muscle) |
属性
| 形式 | Liquid |
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| 缓冲液 | PBS and 0.1% Sodium azide. |
| 抗菌剂 | 0.1% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 背景介绍 | This antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells. Actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), and myocardium (alpha-myocardial) are not stained. |
| 细胞定位 | Cytoplasm |
| 产品亮点 | Related Antibody Duos and Panels: ARG30293 Smooth Muscle Marker Related products: alpha smooth muscle Actin antibodies; alpha smooth muscle Actin Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels for Myofibroblasts and CAFs Besides tumor suppression, what’s p53 busy for during embryogenesis? New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy |
| 研究领域 | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody |
| 预测分子量 | 42 kDa |
| 翻译后修饰 | Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). |
检测图片 (3) Click the Picture to Zoom In
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ARG52485 anti-alpha smooth muscle Actin antibody [1A4] ICC/IF image
Immunofluorescence: Rabbit skeletal muscle stained with ARG52485 anti-alpha smooth muscle Actin antibody [1A4] at 1:100 dilution.
Secondary antibody: ARG23757 Goat anti-Mouse IgG (H+L) antibody (FITC), pre-adsorbed
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ARG52485 anti-alpha smooth muscle Actin antibody [1A4] WB image
Western blot: 30 µg of MEF cell lysate stained with anti-alpha smooth muscle Actin antibody [1A4] at 1:200 dilution.
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ARG52485 anti-Actin-Smooth Muscle antibody [1A4] IHC-P image
Immunohistochemistry: Human Small Intestine stained with Actin-Smooth Muscle antibody [1A4] (ARG52485)
客户反馈
Excellent
anti-alpha smooth muscle Actin antibody [1A4]
Application：IF/ICC
Sample：Rabbit skeletal muscle
Fixation Buffer：4% PFA
Fixation Time：20 min
Fixation Temperature：25 ºC
Antigen Retrieval Buffer：-
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：Overnight
Primary Antibody Incubation Temperature：4 ºC
Good
anti-alpha smooth muscle Actin antibody [1A4]
Application：WB
Sample：Rat heart
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:300
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-alpha smooth muscle Actin antibody [1A4]
Application：WB
Sample：MEF cell lysate
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
Comparative Assessment of Short-Term Tendon-Scleral Postoperative Inflammation and α-Smooth Muscle Actin Expression following Oral and Topical Diclofenac Administration for Strabismus Surgery in Rabbits
IHC-P / Rabbit
Pleiotropic Long-Term Effects of Atorvastatin on Posttraumatic Joint Contracture in a Rat Model
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Acceleration of ligamentization and osseointegration processes after anterior cruciate ligament reconstruction with autologous tissue-engineered polyethylene terephthalate graft.
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MicroRNA-145 Delay Plaque Erosion by Down-Regulating TGFβr Ii and Suppressing Autophagy of Vascular Smooth Muscle Cells.
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Inhibiting necroptosis of spermatogonial stem cell as a novel strategy for male fertility preservation.
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Effects of losartan and atorvastatin on the development of early posttraumatic joint stiffness in a rat model.
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Establishing a nonlethal and efficient mouse model of male gonadotoxicity by intraperitoneal busulfan injection.
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Preparation and renoprotective effects of carboxymethyl chitosan oligosaccharide on adriamycin nephropathy
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Macrophage migration inhibitory factor is critical for dengue NS1-induced endothelial glycocalyx degradation and hyperpermeability
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