ARG63177
anti-c-Cbl antibody
anti-c-Cbl antibody for Western blot and Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes c-Cbl |
|---|---|
| 反应物种 | Ms |
| 预测物种 | Hu, Rat, Cow, Dog, Pig |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | c-Cbl |
| 抗原物种 | Human |
| 抗原 | C-REFVSISSPAHVAT |
| 偶联标记 | Un-conjugated |
| 別名 | Signal transduction protein CBL; C-CBL; EC 6.3.2.-; FRA11B; Casitas B-lineage lymphoma proto-oncogene; Proto-oncogene c-Cbl; RNF55; CBL2; E3 ubiquitin-protein ligase CBL; RING finger protein 55; NSLL |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Mar 2012] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 100 kDa |
| 翻译后修饰 | Phosphorylated on tyrosine residues by ALK, EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues in response to FLT1 and KIT signaling. Phosphorylated on tyrosine residues by INSR and FGR. Phosphorylated on several tyrosine residues by constitutively activated FGFR3. Not phosphorylated at Tyr-731 by FGFR3. Phosphorylated on tyrosine residues by activated CSF1R, PDGFRA and PDGFRB. Phosphorylated on tyrosine residues by HCK. Ubiquitinated, leading to its degradation via the proteasome. |
检测图片 (1) Click the Picture to Zoom In
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ARG63177 anti-c-Cbl antibody WB image
Western blot: 3T3 lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63177 anti-c-Cbl antibody at 2 µg/ml dilution.
