ARG54961
anti-cGAS antibody
anti-cGAS antibody for Flow cytometry,Western blot and Human
Cell Biology and Cellular Response antibody
参考文献1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes cGAS |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | cGAS |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 266-295 (Center) of Human cGAS. |
| 偶联标记 | Un-conjugated |
| 別名 | cGAMP synthase; Cyclic GMP-AMP synthase; Mab-21 domain-containing protein 1; C6orf150; EC 2.7.7.86; cGAS; h-cGAS |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | MCF7 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MB21D1 |
| 全名 | Mab-21 domain containing 1 |
| 生物功能 | Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production. [UniProt] |
| 细胞定位 | Cytoplasm, cytosol |
| 产品亮点 | Related products: cGAS antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| 研究领域 | Cell Biology and Cellular Response antibody |
| 预测分子量 | 59 kDa |
| 翻译后修饰 | Polyglutamylated by TTLL6 at Glu-286, leading to impair DNA-binding activity. Monoglutamylated at Glu-314 by TTLL4, leading to impair the nucleotidyltransferase activity. Deglutamylated by AGBL5/CCP5 and AGBL6/CCP6. Cleaved by CASP1 at Asp-140 and Asp-157 upon DNA virus infection; the cleavage impairs cGAMP production (PubMed:28314590). Also cleaved by the pyroptotic CASP4 and CASP5 during non-canonical inflammasome activation; they don't cut at the same sites than CASP1 (PubMed:28314590). |
检测图片 (1) Click the Picture to Zoom In
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ARG54961 anti-cGAS antibody WB image
Western blot: 20 µg of MCF7 cell lysate stained with ARG54961 anti-cGAS antibody at 1:1000 dilution.
文献引用
The DNA sensor cGAS is decorated by acetylation and phosphorylation modifications in the context of immune signaling.
WB / Human
