ARG66856
anti-eNOS antibody
anti-eNOS antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Mouse Monoclonal antibody recognizes eNOS |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG |
| 靶点名称 | eNOS |
| 抗原物种 | Human |
| 抗原 | Purified recombinant fragment of Human eNOS. |
| 偶联标记 | Un-conjugated |
| 別名 | Constitutive NOS; NOS type III; Nitric oxide synthase, endothelial; Endothelial NOS; eNOS; EC-NOS; NOSIII; cNOS; EC 1.14.13.39; ECNOS |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | Ascitic fluid, 0.03% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.03% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P29474 Human Nitric oxide synthase, endothelial |
|---|---|
| 基因名称 | NOS3 |
| 全名 | nitric oxide synthase 3 (endothelial cell) |
| 背景介绍 | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
| 生物功能 | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. [Isoform eNOS13C]: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. [UniProt] |
| 细胞定位 | Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. [UniProt] |
| 预测分子量 | 133 kDa |
| 翻译后修饰 | Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG66856 anti-eNOS antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lymph node tissue stained with ARG66856 anti-eNOS antibody.
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ARG66856 anti-eNOS antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue stained with ARG66856 anti-eNOS antibody.
