ARG62989

anti-gamma Tubulin antibody [TU-30]

Datasheet

订购信息

包装

价格 (USD)

100 μg

365

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody [TU-30] recognizes gamma Tubulin
反应物种	Hu, Ms, Rat, Bov, Chk, Pig, Plnt, Prt
应用	FACS, ICC/IF, IHC-P, WB
特异性	The clone TU-30 recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa 434-449) of gamma-Tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). This clone reacts gamma-tubulin 1 but not on human gamma-tubulin 2.
宿主	Mouse
克隆	Monoclonal
克隆号	TU-30
同位型	IgG1
靶点名称	gamma Tubulin
抗原	C-terminal peptide of gamma-Tubulin counjugated to KLH.
抗原表位	The epitope was located in the amino acid sequence TRPDYI (aa439-444 in human), which is present on human gamma-tubulin 1 but not on human gamma-tubulin 2.
偶联标记	Un-conjugated
別名	GCP-1; CDCBM4; TUBG; Gamma-1-tubulin; Tubulin gamma-1 chain; TUBGCP1; Gamma-tubulin complex component 1

应用说明

应用建议

应用	推荐稀释比
FACS	Assay-dependent
ICC/IF	1 - 2 µg/ml
IHC-P	1:500 - 1:1000
WB	1:500 - 1:2000

应用说明

ICC/IF: Staining technique: (a) Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Incubation: 45 min at RT. Note: The clone TU-30 stains only fixed cells.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

阳性对照

ICC/IF: P-19 Mouse embryonal carcinoma cell line and 3T3 Mouse fibroblasts.

属性

形式	Liquid
纯化	Purified from hybridoma culture supernatant by protein-A affinity chromatography.
纯度	> 95% (by SDS-PAGE)
缓冲液	PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂	15 mM Sodium azide
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 103733 Mouse TUBG1 GeneID: 252921 Rat TUBG1 GeneID: 541101 Bovine TUBG1 GeneID: 7283 Human TUBG1 Swiss-port # P23258 Human Tubulin gamma-1 chain Swiss-port # P83887 Mouse Tubulin gamma-1 chain Swiss-port # P83888 Rat Tubulin gamma-1 chain Swiss-port # Q0VCD2 Bovine Tubulin gamma-1 chain More
基因名称	TUBG1
全名	tubulin, gamma 1
背景介绍	The gamma-Tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of Tubulin family (less that 0.01% of Tubulin dimer). The gamma-Tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-Tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.
生物功能	Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation. [UniProt]
产品亮点	Related products: gamma Tubulin antibodies; Anti-Mouse IgG secondary antibodies; Related news: Gene therapy for retinitis pigmentosa (RP) Related poster download: Organelle Markers & Loading Control
研究领域	Signaling Transduction antibody
预测分子量	51 kDa
翻译后修饰	Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.

检测图片 (4) Click the Picture to Zoom In

ARG62989 anti-gamma Tubulin antibody [TU-30] WB image

Western blot: Human gamma Tubulin isotypes. (A) SH-SY5Y total cell lysate, expressing TagRFP-tagged γ-Tb1 or γ-Tb2. (B) GST-tagged C-terminal region (aa. 362-451) of γ-Tb1 or γ-Tb2. The blots were stained with ARG62989 anti-gamma Tubulin antibody [TU-30].
ARG62989 anti-gamma Tubulin antibody [TU-30] ICC/IF image

Immunofluorescence: P19X1 mouse embryonal carcinoma cells stained with ARG62989 anti-gamma Tubulin antibody [TU-30] (red).
Cell nuclei was stained with DAPI (blue).
ARG62989 anti-gamma Tubulin antibody [TU-30] ICC/IF image

Immunofluorescence: Mouse fibroblasts stained with ARG62989 anti-gamma Tubulin antibody [TU-30] (green).
Cell nuclei was stained with DAPI (blue).
ARG62989 anti-gamma Tubulin antibody [TU-30] ICC/IF image

Immunofluorescence: 3T3 mouse fibroblasts stained with ARG62989 anti-gamma Tubulin antibody [TU-30] (red).
Co-stained with alpha-tubulin (green) and DAPI (blue). A - metaphase; B - anaphase; C - telophase.

克隆号文献

Interactome analysis of the influenza A virus transcription/replication machinery identifies protein phosphatase 6 as a cellular factor required for efficient virus replication.

York A et al.
J Virol., (2014)

Selective impairment of a subset of Ran-GTP-binding domains of ran-binding protein 2 (Ranbp2) suffices to recapitulate the degeneration of the retinal pigment epithelium (RPE) triggered by Ranbp2 ablation.

Patil H et al.
J Biol Chem., (2014)

Mitotic phosphorylation of histone H3 threonine 80.

Hammond SL et al.
Cell Cycle., (2014)

Plus-end tracking proteins, CLASPs, and a viral Akt mimic regulate herpesvirus-induced stable microtubule formation and virus spread.

Naghavi MH et al.
Proc Natl Acad Sci U S A., (2013)

Mitosis phase enrichment with identification of mitotic centromere-associated kinesin as a therapeutic target in castration-resistant prostate cancer.

IHC / Human

Sircar K et al.
PLoS One., (2012)

Kif7 promotes hedgehog signaling in growth plate chondrocytes by restricting the inhibitory function of Sufu.

IHC-P / Mouse

Hsu SH et al.
Development., (2011)

WDR62 is associated with the spindle pole and is mutated in human microcephaly.

IHC-P / Human

Nicholas AK et al.
Nat Genet., (2010)

Discovery of a distinct domain in cyclin A sufficient for centrosomal localization independently of Cdk binding.

ICC/IF / Human, Chinese Hamster, Xenopus laevis

Pascreau G et al.
Proc Natl Acad Sci U S A., (2010)

Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells.