ARG10759
anti-mCherry antibody [1C51]
anti-mCherry antibody [1C51] for ICC/IF,IHC-Frozen sections,Western blot and Other
概述
| 产品描述 | Mouse Monoclonal Antibody to mCherry |
|---|---|
| 反应物种 | Other |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1C51 |
| 同位型 | IgG2a |
| 靶点名称 | mCherry |
| 抗原物种 | Others |
| 抗原 | Full length recombinant protein. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification. |
| 缓冲液 | PBS and 50% Glycerol. |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
检测图片 (3) Click the Picture to Zoom In
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ARG10759 anti-mCherry antibody [1C51] ICC/IF image
Immunocytochemistry: HEK293 cells transfected with mCherry and visualized in red. The cells were stained with ARG10759 anti-mCherry antibody [1C51] in green, and visualized using a confocal microscope. Transfected cells are yellow, showing overlap of the mCherry and the ARG10759 anti-mCherry antibody [1C51]. Untransfected HEK293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue).
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ARG10759 anti-mCherry antibody [1C51] WB image
Western blot: Crude extract of HEK293 cells transfected with pFin-EF1-mCherry vector in lane labelled "+". The "-" lane is a blot of an equal amount of protein extract from untransfected HEK293 cells. ARG10759 anti-mCherry antibody [1C51] binds a major band running at ~28 kDa corresponding to intact full-length mCherry. The two other bands are clearly processed forms of mCherry as they are not present in non-transfected HEK293 cells.
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ARG10759 anti-mCherry antibody [1C51] WB image
Western blot: 1) HEK293 (non-transfected), 2) HEK293 transfected with mCherry-HA construct, 3) Recombinant mCherry protein, 4) Recombinant GFP protein and 5) HEK293 transfected with GFP construct. The blots were stained with ARG10759 anti-mCherry antibody [1C51] (green) at 1:1000 dilution. The same blot was simultaneously stained with ARG10757 anti-Hsp 60 antibody (red) at 1:5000 dilution.
