ARG66653
anti-p15 INK4b antibody
anti-p15 INK4b antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes p15 INK4b |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | p15 INK4b |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide between aa. 60-140 of Human p15 INK4b. |
| 偶联标记 | Un-conjugated |
| 別名 | MTS-2; MTS2; Multiple tumor suppressor 2; p14-INK4b; Cyclin-dependent kinase 4 inhibitor B; TP15; p15INK4B; p15-INK4b; P15; p15INK4b; CDK4I; INK4B |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: High-pressure and temperature EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 15 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P42772 Human Cyclin-dependent kinase 4 inhibitor B Swiss-port # P55271 Mouse Cyclin-dependent kinase 4 inhibitor B |
|---|---|
| 基因名称 | CDKN2B |
| 全名 | cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) |
| 背景介绍 | This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008] |
| 生物功能 | Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest. [UniProt] |
| 细胞定位 | Cytoplasm. Note=Also found in the nucleus. [UniProt] |
| 预测分子量 | 15 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG66653 anti-p15 INK4b antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG66653 anti-p15 INK4b antibody. The picture on the right is blocked with the synthetic peptide.
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ARG66653 anti-p15 INK4b antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: High-pressure and temperature EDTA buffer (pH 8.0). Tissue section was stained with ARG66653 anti-p15 INK4b antibody at 1:100 dilution (4°C, overnight).
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ARG66653 anti-p15 INK4b antibody WB image
Western blot: HeLa cell lysate stained with ARG66653 anti-p15 INK4b antibody at 1:500 dilution.
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ARG66653 anti-p15 INK4b antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: High-pressure and temperature EDTA buffer (pH 8.0). Tissue section was stained with ARG66653 anti-p15 INK4b antibody at 1:100 dilution (4°C, overnight).
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ARG66653 anti-p15 INK4b antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung carcinoma tissue stained with ARG66653 anti-p15 INK4b antibody. The picture on the right is blocked with the synthetic peptide.
