ARG67225
anti-p21 antibody
anti-p21 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes p21 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | p21 |
| 偶联标记 | Un-conjugated |
| 別名 | CDKN1A; Cyclin Dependent Kinase Inhibitor 1A; CAP20; CIP1; WAF1; SDI1; P21; P21CIP1; CDKN1; Cyclin-Dependent Kinase Inhibitor 1A (P21, Cip1); Cyclin-Dependent Kinase Inhibitor 1; Cdk-Interacting Protein 1; P21Cip1/Waf1; MDA-6; Melanoma Differentiation Associated Protein 6; Melanoma Differentiation-Associated Protein 6; Wild-Type P53-Activated Fragment 1; CDK-Interaction Protein 1; CDK-Interacting Protein 1; DNA Synthesis Inhibitor; MDA6; PIC1 |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol. |
| 抗菌剂 | 0.025% ProClin 300 |
| 稳定剂 | 20% Glycerol |
| 浓度 | 0.3 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P38936 Human Cyclin-dependent kinase inhibitor 1 Swiss-port # P39689 Mouse Cyclin-dependent kinase inhibitor 1 |
|---|---|
| 基因名称 | CDKN1A |
| 全名 | Cyclin Dependent Kinase Inhibitor 1A |
| 背景介绍 | This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015] |
| 生物功能 | Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes. [Uniprot] |
| 细胞定位 | Cytoplasm, Nucleus. [Uniprot] |
| 预测分子量 | 18 kDa |
| 翻译后修饰 | Acetylation, Phosphoprotein, Ubl conjugation. [Uniprot] |
检测图片 (3) Click the Picture to Zoom In
-
ARG67225 anti-p21 antibody IHC-P image
Immunohistochemistry: Human testis carcinoma stained with ARG67225 anti-p21 antibody at 1:100 dilution.
-
ARG67225 anti-p21 antibody ICC/IF image
Immunofluorescence: HT-29 stained with ARG67225 anti-p21 antibody at 1:400 dilution.
-
ARG67225 anti-p21 antibody IHC-P image
Immunohistochemistry: Human colorectal carcinoma stained with ARG67225 anti-p21 antibody at 1:100 dilution.
