ARG63503
anti-p70 S6 Kinase antibody
anti-p70 S6 Kinase antibody for Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes p70 S6 Kinase |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow, Rat, Dog |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | p70 S6 Kinase |
| 抗原物种 | Human |
| 抗原 | C-MISKRPEHLRMNL |
| 偶联标记 | Un-conjugated |
| 別名 | p70 S6 kinase alpha; p70(S6K)-alpha; p70-alpha; p70 S6KA; S6K; STK14A; S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; 70 kDa ribosomal protein S6 kinase 1; S6K-beta-1; P70S6K1; PS6K; p70 ribosomal S6 kinase alpha; p70-S6K; EC 2.7.11.1; Serine/threonine-protein kinase 14A; p70 S6K-alpha; Ribosomal protein S6 kinase beta-1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P23443 Human Ribosomal protein S6 kinase beta-1 Swiss-port # Q8BSK8 Mouse Ribosomal protein S6 kinase beta-1 |
|---|---|
| 背景介绍 | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 59 kDa |
| 翻译后修饰 | Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (By similarity). Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion. |
检测图片 (2) Click the Picture to Zoom In
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ARG63503 anti-p70 S6 Kinase antibody WB image
Western blot: 35 µg of Human placenta lysate (in RIPA buffer) stained with ARG63503 anti-p70 S6 Kinase antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG63503 anti-p70 S6 Kinase antibody WB image
Western blot: 35 µg of NIH/3T3 cell lysate (in RIPA buffer) stained with ARG63503 anti-p70 S6 Kinase antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
