ARG83521
arigoQIK® Mouse VEGF ELISA Kit
arigoQIK® Mouse VEGF ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG83521-01 | Antibody Coated Microplate | 8 X 12 strips | 4°C |
| ARG83521-02 | Standard | 2 vials | 4°C |
| ARG83521-03 | 1000X HRP-Streptavidin solution | 15 μl | 4°C |
| ARG83521-04 | Standard/Sample Diluent Buffer | 20 ml | 4°C |
| ARG83521-05 | HRP Diluent Buffer | 20 ml | 4°C |
| ARG83521-06 | 10X Wash Buffer | 35 ml | 4°C |
| ARG83521-07 | TMB substrate | 12 ml | 4°C (protect from light) |
| ARG83521-08 | STOP solution | 12 ml | 4°C |
| ARG83521-09 | Plate sealer | 2 strips | Room temperature |
概述
| 产品描述 | ARG83521 arigoQIK® Mouse VEGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse VEGF serum, plasma and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | VEGF |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 11.71 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 23.44 - 1500 pg/ml |
| 样本量 | 50 μL |
| 精确 | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| 別名 | EGFA; Vascular Endothelial Growth Factor A; VPF; VEGF; Vascular Endothelial Growth Factor A, Long Form; Vascular Permeability Factor; VEGF-A; L-VEGF; Vascular Endothelial Growth Factor A121; Vascular Endothelial Growth Factor A165; Vascular Endothelial Growth Factor; MVCD1 |
应用说明
| 检测时间 | 2 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 4°C, -20°C, -80°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q00731 Mouse Vascular endothelial growth factor A |
|---|---|
| 基因名称 | Vascular Endothelial Growth Factor A |
| 全名 | VEGFA |
| 背景介绍 | This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces prolifeMouseion and migMouseion of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. The levels of VEGF are increased during infection with severe acute respiMouseory syndrome coronavirus 2 (SARS-CoV-2), thus promoting inflammation by facilitating recruitment of inflammatory cells, and by increasing the level of angiopoietin II (Ang II), one of two products of the SARS-CoV-2 binding target, angiotensin-converting enzyme 2 (ACE2). In turn, Ang II facilitates the elevation of VEGF, thus forming a vicious cycle in the release of inflammatory cytokines. [provided by RefSeq, Jun 2020] |
| 生物功能 | Induces endothelial cell prolifeMouseion, promotes cell migMouseion, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migMouseion, including for the caudal migMouseion of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. [Uniprot] |
| 细胞定位 | Cytoplasm, Endoplasmic reticulum, Extracellular matrix, Golgi appaMouseus, Nucleus, Secreted. [Uniprot] |
检测图片 (1) Click the Picture to Zoom In
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ARG83521 arigoQIKTM Mouse VEGF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
ARG83521 arigoQIKTM Mouse VEGF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| arigoQIKTM Mouse VEGF ELISA Kit User manual |
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